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. 2013 Jul 29;2:138. Originally published 2013 Jun 10. [Version 2] doi: 10.12688/f1000research.2-138.v2

Table 3. List of primers used for amplifying CHLI1, four neighboring genes upstream of CHLI1 and the actin gene and transcript.

These primers were used to generate the data in Figure 6B and 6C. The gene loci in Phytozome ( http://www.phytozome.net/) are: CHLI1 (Cre06.g306300), FDX3 (Cre06.g306350), AMT (g7098), UP5 (Cre06.g306450) and UP6 (Cre06.g306500) and Actin (Cre13.g603700).

Primer name Sequence of primer Gene
CHLI1AF 5´-ACTACGACTTCCGCGTCAAGATCA-3´ CHLI1
CHLI1BR 5´-CATGCCGAACACCTGCTTGAAGAT-3´ CHLI1
PB134 5´-CTGGAGCGCACCTTTATGAAG-3´ AMT
PB135 5´-AGTGGAACAGGTTCTCGATGAC-3´ AMT
PB132 5´-AAGGGCATCAGCTACAAGGTC-3´ FDX3
PB133 5´-GGCATCGAGGATGTATTGGTTG-3´ FDX3
UP5F 5´-GGG CAA CTG GAG CTT TGG C-3´ UP5
UP5R 5´-CGT CTA TGT GCG CCA CGT C-3´ UP5
UP6F 5´-GCA ACT GGA GCT TCG GCG-3´ UP6
UP6R 5´-CGT AGG CGC CAA ACA CCG-3´ UP6
F2 5´-ACGACACCACCTTCAACTCCATCA-3´ Actin
R2 5´-TTAGAAGCACTTCCGGTGCACGAT-3´ Actin