Table 3. List of primers used for amplifying CHLI1, four neighboring genes upstream of CHLI1 and the actin gene and transcript.
Primer name | Sequence of primer | Gene |
---|---|---|
CHLI1AF | 5´-ACTACGACTTCCGCGTCAAGATCA-3´ | CHLI1 |
CHLI1BR | 5´-CATGCCGAACACCTGCTTGAAGAT-3´ | CHLI1 |
PB134 | 5´-CTGGAGCGCACCTTTATGAAG-3´ | AMT |
PB135 | 5´-AGTGGAACAGGTTCTCGATGAC-3´ | AMT |
PB132 | 5´-AAGGGCATCAGCTACAAGGTC-3´ | FDX3 |
PB133 | 5´-GGCATCGAGGATGTATTGGTTG-3´ | FDX3 |
UP5F | 5´-GGG CAA CTG GAG CTT TGG C-3´ | UP5 |
UP5R | 5´-CGT CTA TGT GCG CCA CGT C-3´ | UP5 |
UP6F | 5´-GCA ACT GGA GCT TCG GCG-3´ | UP6 |
UP6R | 5´-CGT AGG CGC CAA ACA CCG-3´ | UP6 |
F2 | 5´-ACGACACCACCTTCAACTCCATCA-3´ | Actin |
R2 | 5´-TTAGAAGCACTTCCGGTGCACGAT-3´ | Actin |