Figure 4. Effect of Continuous Exposure to PTHrP (1-36) On Bone Nodules and AP-Positive Osteogenic Cell Colonies In Low Plating Density Cultures.

RC cells were exposed to 0 to 100 nM PTHrP (1-36) for 18 days and then fixed and stained by the von Kossa method to identify mineralized bone nodules ( A). or histochemically stained to identify AP-positive cell colonies ( B). Results are the mean±SEM (n=3), **P≤0.01, and ***P≤0.001.