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. 2013 Jun 13;2:77. Originally published 2013 Mar 5. [Version 2] doi: 10.12688/f1000research.2-77.v2

Figure 8. Effect of Intermittent and Continuous Exposure to PTHrP (1-36) on BSP and OPN mRNA and BSP Protein.

Figure 8.

The BSP ( A) and OPN ( B) mRNA in RC cells exposed to 100 nM PTHrP (1-36) for 1 hr, 6 hrs, or 48 hrs for seven 48 hr treatment cycles (15 days) was measured by real-time RTPCR. The effect of 0.01 and 100 nM PTHrP (1-36) on BSP in lysates of RC cells was assessed by immunoblotting ( C). Continuous exposure to 100 nM PTHrP (1-36) for 14 d decreased immunodetectable 75 kD BSP compared to the control and 0.01 nM PTHrP (1-36) treated RC cells. The Ponceau S-stained gel shows relative amounts of loaded protein. Real-time RTPCR results are the mean±SEM (n=3), ***P≤0.001.