Table 1.
Prediction of miR-200 binding sites on FN1, ZEB1, ZEB2, KRT7, KRT8 and KRT18 mRNAs
| miRNA | miR-200b | miR-200c | miR-429 | miR-200a | miR-141 | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
Prediction algorithm |
†A |
¥B |
ǂC |
A |
B |
C |
A |
B |
C |
A |
B |
C |
A |
B |
C |
|
FN1 |
2 |
2 |
1 |
2 |
2 |
1 |
2 |
2 |
1 |
0 |
0 |
0 |
0 |
0 |
0 |
|
ZEB1 |
5 |
6 |
5 |
5 |
6 |
5 |
5 |
6 |
5 |
3 |
3 |
3 |
3 |
3 |
3 |
|
ZEB2 |
6 |
5 |
6 |
6 |
5 |
6 |
6 |
5 |
6 |
3 |
4 |
2 |
3 |
4 |
2 |
|
KRT7 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
|
KRT8 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
| KRT18 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Only those genes predicted to be targeted by miR-200 family members were observed to be downregulated in cells in which they were ectopically overexpressed.
†A: miRanda (mirSVR ≤ -0.2), ¥B: miRDB, ǂC: TargetScan (conserved miRNA sites).