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. Author manuscript; available in PMC: 2014 Jan 24.
Published in final edited form as: Mol Cancer Res. 2011 May 6;9(6):766–781. doi: 10.1158/1541-7786.MCR-10-0317

Figure 4.

Figure 4

Effect of NFBD1 depletion on focal adhesions and actin cytoskeleton. A–C, U2OS cells were infected with control, Cav1, or NFBD1a shRNA-expressing retroviruses. A and B, two days after infection, cells were plated on glass chamber slides coated with fibronectin. D, NFBD1+/+ and NFBD1−/− MEFs were plated on fibronectin-coated glass chamber slides. A, B, D, immunofluorescence was carried out using vinculin, NFBD1, or Cav1 antibodies. Actin was stained with Texas Red–phalloidin. C, western blots were carried out on lysates using the indicated antibodies.