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. Author manuscript; available in PMC: 2014 Jan 24.
Published in final edited form as: Mol Cancer Res. 2010 Sep 21;8(10):1388–1398. doi: 10.1158/1541-7786.MCR-10-0042

FIGURE 3.

FIGURE 3

Effect of ErbB4 kinase activity and ICD isoform on interaction with Kap1. Truncated V5-tagged ErbB4 isoforms CYT-1, kinase-deficient CYT-1 (CYT-1 KD), CYT-2, or CYT-2 KD were transiently coexpressed with Flag-tagged Kap1 in COS-7 cells that were then stimulated with 100 ng/mL TPA. Protein extracts were immunoprecipitated using antibodies against the epitope tags and immunoblotted as marked. Portions of the lysates were analyzed directly by immunoblotting for V5, Flag, and GAPDH without immunoprecipitation. Data represent two biological replicates in identical format plus two similar biological replicates.