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. Author manuscript; available in PMC: 2014 Jan 24.
Published in final edited form as: Mol Cancer Res. 2010 Sep 21;8(10):1388–1398. doi: 10.1158/1541-7786.MCR-10-0042

FIGURE 6.

FIGURE 6

MDM2 and ErbB4 s80 affect DNA damage response. MCF-7 control cells (A), MCF-7 cells stably infected with pBabe CYT-2 s80 (B), MCF-7 cells transiently infected with pSiren shMDM2 (C), MCF-7 cells stably with pBabe and transiently infected with pSiren (D), and MCF-7 stably infected with pBABE CYT-2 s80 and transiently infected with pSiren shMDM2 (E) were treated with 10 µmol/L camptothecin for the times indicated (hours). Total protein was extracted using 2× Laemmli sample buffer and proteins were detected by immunoblotting filter strips as indicated. The strips used for blotting of phospho-Kap1 were stripped once and reprobed for MDM2 and then restripped and probed for Kap1. Data represent three biological replicates in identical format plus two similar biological replicates.