Table 3.

Bone histomorphometry measurements performed in the proximal tibia of the offspring from the control group and the mothers subjected to immobilization stress in gestation week 3 (GW3).

	4 weeks				8 weeks				12 weeks				16 weeks
	Control	GW3	Estimated difference adjusted for sex	p value	Control	GW3	Estimated difference adjusted for sex	p value	Control	GW3	Estimated difference adjusted for sex	p value	Control	GW3	Estimated difference adjusted for sex	p value
Number	5	7			8	13			12	8			18	8
Total tissue area (mm2)	4.55	4.36	−0.18	0.788	6.27	6.42	0.4	0.3	7.03	6.61	0.14	0.733	6.77	7.28	0.22	0.733
Bone area (mm2)	1.06	1.25	0.11	0.664	1.4	2.16	0.82***	0.001	1.27	1.61	0.3	0.16	1.39	1.53	0.17	0.58
Bone perimeter (mcm)	41.39	38.62	−3.77	0.714	54.18	60.74	7.4	0.288	47.27	44.43	−2.51	0.779	44.53	47.68	5.02	0.542
Osteoblast surface (mm)	9.59	8.88	−1.48	0.61	11.31	16.8	5.91	0.144	12.14	21.66	11.60*	0.04	10.28	14.92	3.62	0.255
Osteoid surface (mm)	0.42	2.18	1.44	0.093	1.92	4.98	3.15*	0.046	0.99	5.5	4.94*	0.022	1.9	3.12	0.98	0.446
N.Oc per eroded surface (N.Oc/mm)	45.18	245.72	233.31	0.077	95.32	120.99	26.8	0.52	89.26	102.95	−29.07	0.735	74.18	66.57	−9.05	0.829
Eroded surface (mm)	1.46	0.92	−0.64	0.074	0.94	0.81	−0.07	0.597	1.31	0.87	−0.33	0.359	0.98	0.72	−0.22	0.329
Proliferative zone growth plate (mcm)	302.83	210.29	−92.78*	0.023	195.73	123.56	−63.97	0.063	166.01	150.84	−8.67	0.485	130.73	123.35	−10.93	0.305
Hypertrophic zone growth plate (mcm)	179.64	126.15	−48.66	0.244	88.81	70.4	−15.06	0.405	75.44	75.02	1.78	0.735	62.14	62.62	−0.46	0.89

GWS = gestation week 3; N.Oc = Osteoclast Number;

^*= p < 0.05;

^**= p < 0.01;

^***= p< 0.001