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. Author manuscript; available in PMC: 2014 Jan 24.
Published in final edited form as: Adv Exp Med Biol. 2010;664:29–37. doi: 10.1007/978-1-4419-1399-9_4

Fig. 4.2.

Fig. 4.2

Subcellular localization of PEDF-R in retinal cells. Cell lysates (Lys) were fractionated by high speed centrifugation (80,000×g) into cytosolic (cyt) and membrane fractions (Mb). Western blots of fractions were immunostained with anti-PEDF-RA antibody. Total protein loaded in lanes were as follows: (ARPE-19, Lys) 19.8 μg; (ARPE-19, Cyt) 12 μg; (ARPE-19, Mb) 6.5 μg; (RPE, Cyt) 67 μg; (RPE, Mb) 10 μg; (Retina, Cyt) 71 μg; (Retina, Mb) 10 μg; (RGC-5, Mb) 9.5 μg; and (R29, Mb) 5.7 μg respectively. The asterisks point to the migration position of PEDF-R