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Figure 5. Transduction of peripheral blood T cells with the LeGO-MP-muFlu-vector construct. Peripheral blood T cells of buffy coat BCBBe3 were transduced with LeGO-MP-muFlu 72 h after activation with CD3/CD28-Dynabeads. Transduction efficiencies were measured 3 d post transduction via FACS, looking at eGFP-, mTCR- and Flu-tetramer-expression and staining, respectively. Numbers indicate percentages of eGFP, mTCR and Flu-tetramer positive cells.
