Figure 6. IL-2-, IFN-γ and TNF-α- secretion by transduced primary human T lymphocytes upon co-culture with the Flu-M vs. SSX-2- peptide. Primary T cells were transduced with LeGO-MP-muFlu or MP91-muFlu 72h after activation of PBMCs with CD3/CD28-Dynabeads. Five days after transduction cells were co-cultured with peptide (Flu-M, SSX-2) pulsed T2 cells and the secretion of different cytokines was analyzed by ELISA. (A–C) Mean values and ranges for two to three independent experiments with two buffy coats (BCBBe3, 4) are shown. (A) IFN-γ, (B) IL-2, (C) TNF-α. (D) IFN-γ cytokine secretion of transduced primary cells showing the same amount of Flu-tetramer positive cells were compared by ELISA. Mean values of 3 different buffy coats and two experiments are shown. n.d., not detectable.