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. Author manuscript; available in PMC: 2014 Dec 23.
Published in final edited form as: ACS Nano. 2013 Nov 20;7(12):10597–10611. doi: 10.1021/nn404719c

Figure 4. Effect of surface functionalization with NSM on internalization of carriers.

Figure 4

(A) Endocytosis of 1-μm anti-M6PR/IgG carriers or anti-M6PR/NSM carriers (displaying either 80:20 or 50:50 surface-coverage ratios) incubated with live, activated HUVECs for 3 h at 37°C. Phase contrast shows total carriers bound and internalized. Fluorescence images show surface-bound, non-internalized carriers. Arrowheads mark non-internalized carriers and arrows mark endocytosed ones. Scale bar = 10 μm. (B) Total number of carriers internalized per cell. (C) Percent of carriers internalized out of the total numbers of carriers associated to cells (bound plus internalized). Dashed line in (C) represents anti-ICAM carriers. Mean ± standard error of the mean. *Compared to parent anti-M6PR carriers (continuous line in graph); ^compared to control IgG carriers (dotted line in graph); #compared to anti-M6PR/IgG carriers (p<0.05).