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. Author manuscript; available in PMC: 2014 Dec 23.
Published in final edited form as: ACS Nano. 2013 Nov 20;7(12):10597–10611. doi: 10.1021/nn404719c

Figure 8. Safety of NSM-functionalized carriers.

Figure 8

Activated HUVECs were incubated in the absence or presence of anti-M6PR/NSM carriers or anti-M6PR/IgG carriers (50:50 surface-coverage) at 37°C for 3 h. At this time, as well as after a total time of 8 h or 24 h, cells were incubated with calcein AM (which fluoresces green in live cells) and ethidium homodimer-1 (which labels in red the nuclei of dead cells) to quantify: (A) the total number of cells and (B) the percentage of live cells (% viability) by microscopy. Incubation of cells with H2O2 served as a positive control for apoptosis. The gray horizontal bar in (A) represents the highest and lowest control values across time. A similar bar is shown for (B), yet the variability is too narrow and it appears as a line. Scale bars = 50 μm. Mean ± standard error of the mean. *Compares H2O2 to control (p< 0.05). No difference was found comparing anti-M6PR/IgG carriers or anti-M6PR/NSM carriers to control.