Fig. 8.

Analysis of histopathology in the ventral cochlear nucleus (VCN) shows a massive loss of ANF projections and a more modest loss of VCN volume and neuronal counts. To assess ANF projections, frozen sections of the VCN (A, B) were immunostained with anti-VGLUT1 (red); immunostaining with anti-neurofilament (NF-green) and DAPI (blue) was used to count VCN neurons. C Quantification of the density of ANF terminals (VGLUT1-positive staining intensity). D Quantification of VCN volume. E Quantification of VCN neuronal counts. Each metric (C, D, E) is normalized to the mean result from control cases and is based on data from analysis of three sections from each of six control cases, six ouabain cases at 1-month posttreatment survival and six ouabain cases at 3-month posttreatment survival.