FIG. 5.

Phalloidin permeation into live cells of inner ear involves an active pathway. A Mean fluorescence intensity was measured in five separate 100 × 100 μm regions as depicted in the example image. B We observed more fluorescence in utricles that were treated with phalloidin-CF488A before fixation compared with utricles treated with phalloidin-CF488A after fixation (Student’s t test, p = 0.014). C Example micrograph of utricle treated with 66 nM phalloidin-CF488A and then fixed. D Example confocal micrograph of utricle fixed with paraformaldehyde for 20 min then treated with 66 nM phalloidin-CF488A. Scale bars are 100 μm.