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. 2013 Nov 22;15(1):13–30. doi: 10.1007/s10162-013-0425-9

FIG. 8.

FIG. 8

P2Y receptor subunit expression and effects of P2Y6, PLC, and PKC inhibitors on fluorophore-conjugated phalloidin uptake (A) RT-PCR amplification of P2Y receptors in the sensory epithelium and stroma of P5 mouse utricles. The 100 bp ladder shown. B 500 nM MRS 2578, a P2Y6 receptor antagonist, decreased fluorophore-conjugated phalloidin uptake (n = 4). C Summary of effect of U-73122 (PLC antagonist) and BIM II (PKC antagonist) on phalloidin-CF488A permeation. We observed decreased fluorescence from phalloidin-CF488A treatment in utricles treated with 10 μM U-73122 (n = 5) or 50 μM BIM II (n = 6). Utricles treated with UDP and either U-73122 (n = 4) or BIM II (n = 4) also had significantly decreased fluorescence compared with controls.