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. Author manuscript; available in PMC: 2015 Jan 20.
Published in final edited form as: Virology. 2013 Dec 12;449:244–253. doi: 10.1016/j.virol.2013.11.029

FIG. 1.

FIG. 1

EMSA and supershift assays using 40 bp oligonucleotides containing the wild-type YY1 site of the ORF10 promoter. A) Supershift assays were done in the presence of VZV-infected cell nuclear extracts using polyclonal antibodies against YY1. B) EMSA assays using wild type and YY1 mutant oligonucleotides. C) EMSA and competition assays using Box A containing oligonucleotides as non specific competitor. Lanes: FO, free oligonucleotide; I, VZV-infected nuclear extract. The positions of the YY1 containing complexes are indicated by arrows and the positions of the supershifted bands are indicated by asterisks. The numbers under the figures represent the relative amounts of YY1 complexes, assessed by densitometry.