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. Author manuscript; available in PMC: 2015 Jan 20.
Published in final edited form as: Virology. 2013 Dec 12;449:244–253. doi: 10.1016/j.virol.2013.11.029

Fig. 5.

Fig. 5

The effect of YY1 site mutation in the ORF28/29 promoter on ORF28 gene expression. A) The nucleotide sequence of the ORF28-ORF29 intergenic region. The YY1 site is boxed. The USF site reported to be important for ORF28 and ORF29 expression is underlined. The TATA boxes appear in bold italics. B and C) The results of triplicate assays assessing the effects of the presence of the YY1 site mutation in the ORF28 promoter on the expression levels of the Renilla luciferase reporter in the context of B) pCMV-ORF62 transfection and C) VZV super-infection. The promoter activities resulting from the presence of transfected ORF62 or VZV super-infection are reported as induction (n-fold) of luciferase activity over basal (without ORF62 transfection or VZV super-infection respectively). Statistical significance was determined by a one-way ANOVA analysis of variance followed by Tukey’s post hoc test. Error bars indicate standard error.