Figure 5.

Inhibition of TNFα stimulated NF-κB activation by a mcirofluidically delivered IκB Kinase (IKK) inhibitor Sc-514. A: iHUVECs stimulated with 10 ng/ml TNFα added to the medium for 1 hour and exposed to different concentrations of Sc-514 into the medium for 2 hours. Approximately 80 cells were measured for each data point. Error bars represent the standard error of the mean. Insets show typical images corresponding to 100 (left) and 0 (right) μM of Sc514; B: iHUVECs stimulated by printed TNFα (50 μg/ml coating concentration) for 1 hour were exposed to a Sc-514 gradient within a microfluidic channel for subsequent 2 hours, with concentrations ranging from 0 to 100 μM. The gradient visualized within the device and one of the channels using Alexa 594 and the typical cell groups captured on the protein spots are shown in the upper part of the panel. The results were binned according to the Sc-514 concentration ranges: ‘low’ (0–33 μM), ‘medium’ (33–66 μM) and ‘high’ (66–100 μM). Approximately 50 cells were measured for each data point, with error bars representing the standard error of the mean. In both (A) and (B) T-test significance is represented by one asterisk, P< 0.05 to 0.01; two asterisks, P< 0.01 to 0.001, three asterisks, P< 0.001.