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. Author manuscript; available in PMC: 2014 Jan 25.
Published in final edited form as: Hepatology. 2010 Mar;51(3):881–890. doi: 10.1002/hep.23381

Figure 6. Enforced expression of IL-6 alters expression of DNMT-1 targeting miRNA in vivo.

Figure 6

IL-6 over-expressing (Mz-IL-6) or control (Mz-1) cells (3×106) were injected subcutaneously into the flank of athymic BALB/c mice. Panel A: Western blot analysis was performed in tissue lysates from tumor cell xenografts using antibodies to DNMT-1, Rassf1a, p16INK4a, and α-tubulin. A representative blot image is shown on left panel whereas the densitometric analysis is shown on right panel. Panel B: miRNA was extracted from xenograft tissues and miR-148a, miR-152 and miR-301 expression was assessed by quantitative real-time PCR. Potential DNMT-1 targeting miRNA expressions were normalized to expression of RNU6B. The data represent results from three experiments performed in triplicate. *p<0.05 when compared with miRNA expression in control cell xenografts.