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Neutralization of charges in segments IS4. Voltage dependences of steady state activation of construct IS4N-1 (K264Q/R267Q/R270Q/R273Q) inducing the leftward ΔV 0.5 = − 5.6 ± 1.1 mV shift compared to wild type (a) and time constants of activation/deactivation (b) of the indicated channel construct. Solid lines represent model simulations based on the identified model parameters (see Table 1)
