Table 1.

Midpoints (V _0.5) of the activation curves and rate constants of four-state model

	V 0.5 (mV)	α	β	γ	δ	K VS (x 0/y 0)
Construct
WT	−18.8 ± 0.9 (n = 9)	0.472 ± 0.002	0.263 ± 0.001	0.020 ± 10−4	1.358 ± 10−4	4.57 ± 0.02
R650Q	−22.9 ± 0.8 (9)	0.219 ± 0.003	0.124 ± 0.002	0.026 ± 0.001	0.550 ± 0.001	9.42 ± 0.17
R653Q	−18.5 ± 0.8 (9)	0.747 ± 10−4	0.235 ± 10−4	0.027 ± 10−4	0.732 ± 10−4	1.82 ± 10−3
R656Q	−21.1 ± 0.8 (6)	0.953 ± 10−4	0.106 ± 10−4	0.041 ± 10−4	0.865 ± 10−4	1.48 ± 10−3
K659Q	−17.7 ± 0.8 (6)	0.364 ± 0.005	0.164 ± 0.002	0.025 ± 10−4	2.230 ± 0.002	1.65 ± 0.03
R662Q	−8.4 ± 1.3 (6)	0.637 ± 10−4	0.247 ± 10−4	0.020 ± 10−4	0.968 ± 10−4	1.07 ± 10−3
IIS4N	−17.5 ± 0.8 (11)	0.629 ± 10−4	0.241 ± 10−4	0.043 ± 10−4	1.176 ± 10−4	1.80 ± 10−3
IS4N-1	−24.4 ± 0.7 (5)	0.267 ± 0.001	0.164 ± 10−4	0.009 ± 10−4	0.453 ±2 × 10−4	5.46 ± 0.03
Pore mutations a
G432W	−33.7 ± 0.6 (8)	0.298 ± 10−4	0.046 ± 10−4	0.0003 ± 10−5	0.431 ± 10−4	30.35 ± 0.04
V433C	−8.2 ± 0.8 (5)	0.483 ± 10−4	0.051 ± 10−4	0.024 ± 10−4	1.001 ± 10−4	0.29 ± 10−3
A780T	−44.0 ± 0.6 (5)	0.254 ± 10−4	0.036 ± 10−4	0.004 ± 10−4	0.329 ± 10−4	67.33 ± 0.15
I781T	−47.1 ± 0.9 (5)	0.389 ± 10−4	0.046 ± 10−4	0.005 ± 10−4	0.687 ± 10−4	23.24 ± 10−3
G1193T	−49.8 ± 1.0 (7)	0.479 ± 0.001	0.060 ± 10−4	0.002 ± 10−4	0.581 ± 0.005	199.79 ± 3.12
F1194C	−6.8 ± 0.9 (5)	1.254 ± 10−4	0.067 ± 10−4	0.110 ± 10−4	0.550 ± 10−4	0.11 ± 10−3
A1503G	−41.4 ± 0.8 (5)	0.452 ± 10−4	0.039 ± 10−4	0.015 ± 10−4	0.451 ± 10−4	27.51 ± 0.07
V1504T	−11.6 ± 0.7 (7)	0.803 ± 10−4	0.063 ± 10−4	0.138 ± 10−4	0.562 ± 10−4	0.57 ± 10−3
Pore mutations/IIS4N a
G432W/IIS4N	−19.3 ± 0.7 (8)	0.425 ± 10−4	0.119 ± 10−4	0.017 ± 10−4	0.608 ± 10−4	2.52 ± 10−3
V433C/IIS4N	−12.4 ± 0.7 (7)	0.801 ± 10−4	0.100 ± 10−4	0.030 ± 10−4	0.757 ± 10−4	0.38 ± 10−3
A780T/IIS4N	−32.0 ± 0.7 (10)	0.497 ± 10−4	0.028 ± 10−4	0.003 ± 10−4	0.288 ± 10−4	6.70 ± 10−3
I781T/IIS4N	−54.8 ± 0.6 (9)	0.299 ± 10−4	0.052 ± 10−4	0.006 ± 10−4	0.198 ± 10−4	200
G1193T/IIS4N	−20.8 ± 0.7 (5)	0.465 ± 0.004	0.122 ± 0.001	0.004 ± 10−4	0.804 ± 0.001	2.03 ± 0.03
F1194C/IIS4N	−5.7 ± 0.9 (5)	0.713 ± 10−4	0.100 ± 10−4	0.130 ± 10−4	0.728 ± 10−4	0.24 ± 10−3
A1503G/IIS4N	−19.8 ± 0.7 (6)	0.357 ± 0.002	0.173 ± 0.001	0.003 ± 10−4	0.648 ± 10−4	2.76 ± 0.02
V1504T/IIS4N	−14.0 ± 0.9 (5)	0.531 ± 10−4	0.094 ± 10−4	0.030 ± 10−4	0.537 ± 10−4	1.08 ± 10−3

Numbers of experiments are indicated in parentheses

^a V _0.5 data from Beyl et al. [3]