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. Author manuscript; available in PMC: 2014 Dec 2.
Published in final edited form as: Mol Pharm. 2013 Nov 8;10(12):4462–4471. doi: 10.1021/mp400292p

Figure 4. PTH therapy dramatically affects osteogenic and angiogenic gene expression.

Figure 4

Allografts and adjacent tissues were harvested on Days 3, 7, 10, and 14 after surgery. Gene expression was evaluated with the aid of qRT-PCR by using specific primers for the following: the osteogenic genes alkaline phosphatase (ALP), integrin binding sialoprotein (Ibsp), osteocalcin (Oc/Bglap), and osteopontin (OPN) (A–D); the fibrosis-related gene connective tissue growth factor (CTGF or CCN2) (E); and the angiogenic genes angiopoietin 2 (Angpo-2), vascular endothelial growth factors A and D (VEGF-A and VEGF-D, respectively), and vascular endothelial growth factor receptor 1 (VEGF-R1) (F-I). The gene expression levels were normalized to the expression of the 18S housekeeping gene, n = 5, bars represent ± SE; *p < 0.05.