Skip to main content
. Author manuscript; available in PMC: 2014 Dec 2.
Published in final edited form as: Mol Pharm. 2013 Nov 8;10(12):4462–4471. doi: 10.1021/mp400292p

Figure 6. Mast cell infiltration was affected by PTH therapy, as detected using immunohistofluorescence (IHF) and toluidine blue staining.

Figure 6

Figure 6

Seven days after surgery, specimens were obtained from mice implanted with allografts and treated with PBS (placebo) (A & C) or PTH (B & D). After fixation and slicing, the sections were stained with antibodies against the mast cell markers mast cell protease 1 (MCP1) and mast cell tryptase (MCT) (A & B). Following that, the sections were stained with toluidine blue, which was useful for longitudinal mast cell quantification (C & D). Numbers of mast cells in both groups (E). Mast cells were manually counted in 36 toluidine blue–stained sections obtained from animals at 1, 2, and 3 weeks after surgery. Bars represent ± SE; * p < 0.05.