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. Author manuscript; available in PMC: 2014 Jan 26.
Published in final edited form as: Arch Ophthalmol. 2011 Jan 10;129(5):555–561. doi: 10.1001/archophthalmol.2010.344

Figure 1. Identification of keratocyte nuclei from histologic sections.

Figure 1

Upper Left, Sagittal section of a normal cornea stained with DAPI. Upper Right, The same sagittal section as in A, marked for counting. Keratocyte nuclei were counted by using a manual point and click method. By convention, nuclei were not counted if they crossed the right boundary of the area defined by the yellow box. Lower Left, Sagittal section of a cornea with Fuchs’ endothelial dystrophy. The cornea was edematous with a thickened Descemet membrane. Keratocyte loss was visible in the anterior stroma. Scale bar, 100 μm.