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. Author manuscript; available in PMC: 2014 Dec 5.
Published in final edited form as: Cell. 2013 Dec 5;155(6):1296–1308. doi: 10.1016/j.cell.2013.10.045

Figure 6.

Figure 6

A. Wild type Mtb was used to infect macrophages. On day 1 after infection, macrophages were treated with 6-FABA, and on day 5, cells were lysed and bacteria plated, showing the 6-FABA was bactericidal in macrophages. B. To test the synergy between 6-FABA and IFN-γ, we added 6-FABA and IFN-γ at concentrations that inhibited approximately half of bacterial growth. C. Together, their effect was greater than the estimated additive effect of the two molecules in the absence of synergy, showing that they indeed work synergistically to kill Mtb in macrophages. Mice were infected with 102 aerosolized Mtb bacilli. After 8 days of infection, mice were treated with INH (25 mg/kg), 6-FABA (200 mg/kg) or with the ester derivative of 6-FABA (200 mg/kg). At 2 weeks (D and E) and 4 weeks (F and G) after infection, lungs and spleens were homogenized and plated for CFU. *: P-val < 0.05. **: P-val < 0.01. Insets: linear scale CFU.