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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Biochim Biophys Acta. 2013 Nov 20;1837(2):287–295. doi: 10.1016/j.bbabio.2013.11.007

Figure 4. RE as a function of substrate addition in non-stressed mitochondria.

Figure 4

The experiment was carried out with MCB-loaded [20µM] mitochondria as described in Figure 3. The relative abundance of NAD(P)H (%) and GSH (mM) and H2O2 emission were monitored simultaneously as a function of G/M [A, state 4 respiration] or 5mM G/M plus different concentrations of ADP [B, state 3 respiration], and the RE as a function of G/M [C] and ADP [D] calculated as described under M&M. From the data in panel B, the RE in the abscissa of panel D was calculated; the additional symbols in panel D correspond to interpolated values, e.g. GSH at 400µM ADP. The NAD(P)H signal was calibrated as described under M&M. The arrows in panels C and D indicate increasing substrate addition.