Table 3.
The sequences and information of LAMP primers
| Label | 5′pos | 3′pos | len | Tm | 5′dG | 3′dG | GCrate | Sequence |
|---|---|---|---|---|---|---|---|---|
| F3 |
204 |
222 |
19 |
56.03 |
-5.56 |
-4.06 |
0.42 |
ACCACAATCMACAGTCATT |
| B3 |
398 |
415 |
18 |
55.58 |
-7.53 |
-4.56 |
0.50 |
CCCGCATCATTTGATGTG |
| FIP |
|
|
39 |
|
|
|
|
CAAAGTCGCTTTGCCCTTGG-GATGCTATCTTCCGAAGGA |
| BIP |
|
|
41 |
|
|
|
|
GATCAAACGGCCTGCACAAG-GRAATGTCATTTTGCT RAATG |
| F2 |
243 |
261 |
19 |
55.33 |
-5.14 |
-4.71 |
0.47 |
GATGCTATCTTCCGAAGGA |
| F1c |
291 |
310 |
20 |
61.82 |
-4.16 |
-5.45 |
0.55 |
CAAAGTCGCTTTGCCCTTGG |
| B2 |
377 |
397 |
21 |
55.47 |
-4.51 |
-4.07 |
0.38 |
GR(G or A)AATGTCATTTTGCTRAATG |
| B1c |
326 |
345 |
20 |
61.55 |
-3.92 |
-4.66 |
0.55 |
GATCAAACGGCCTGCACAAG |
| LF |
266 |
286 |
21 |
61.30 |
-6.24 |
-5.45 |
0.48 |
TGCGTTGGTTCAATTCTTGGG |
| LB | 346 | 367 | 22 | 60.11 | -5.26 | -3.73 | 0.45 | GACGTCTTACAACGCAGTAACT |