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. 2014 Jan 15;4(1):29–41.

Figure 7.

Figure 7

IGFBP3 regulates ROS in T.T cells. T.T cells were stably transduced with WT-IGFBP3, GGG-IGFBP3 or empty vector (Bla) to create derivatives with indicated genotypes. A: Cells were subjected to Western blotting with β-actin as a loading control. B: T.T derivatives with indicated genotypes or parental T.T cells incubated with or without rhIGFBP3 (rhBP3) were exposed to either hypoxia or normoxia for 48 h. ROS levels were determined by DCF assays.*, P<0.05 vs. either Bla or 0 nM rhBP3 and normoxia; #, P<0.05 vs. either Bla or 0 nM rhBP3 and hypoxia (n=3).