Table 2.

Influence of PMA on total ceramide (pmol/10⁸ cells), [³H]-labeled ceramide (cpm x 10³/mg wet wt.), sphingosine (pmol/ug protein), and p65 DNA-binding protein of NF-kB (% control) in single aortic smooth muscle cells in the presence and absence of either scyphostatin or fumonisin B1 in low [Mg²⁺]₀-NKRB

	Total ceramide	[3H]-ceramide	sphingosine	p65
Controls (low Mg)	62 ± 5.8*	1.04 ± 0.08*	0.0038 ± 0.0008*	192 ± 16*
PMA (+ low Mg)	82 ± 5.2**	1.32 ± 0.12**	0.0042 ± 0.0010*	198 ± 18*
PMA (+ low Mg) w/scy	76 ± 5.2**	1.28 ± 0.10**	0.0036 ± 0.0008*	188 ± 18*
PMA (+ low Mg) w/fum	74 ± 5.2**	1.08 ± 0.06**	0.0044 ± 0.0010*	146 ± 12*

Values are means ± SE; n=6-8.

^*Significantly different from mean values in normal-NKRB (P<0.01; Table 1).

^**Significantly different from mean values in low [Mg²⁺]₀ (P<0.01).