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. 2014 Jan 15;7(1):22–31.

Figure 1.

Figure 1

Expression of CD40, CD86, dan HLA-DR of DC inhibited by 1,25(OH)2D3. Immature DC taken from monocytes culture supplemented with GM-CSF dan IL-4 for 6 days. On day 7, 1x10-6 cells/well were added with 1,25(OH)2D3 with different doses: 0, 1, 10, 100 nM, then cultured in medium with TNF-α for 4 days. On day 10, cells were harvested and stained with monoclonalantibodies PerCp/Cy5.5 anti-human CD40, FITC anti-human CD86, dan PE anti-human HLA-DR prior to flow cytometry (FACScalibur) analysis. A. Expression of CD40 percentage with different doses of 1,25(OH)2D3. B. Expression of CD86 percentage with different doses of 1,25(OH)2D3. C. Expression of HLA-DR percentage with different doses of 1,25(OH)2D3.