Figure 2.

ASC-CM suppressed H₂O₂- and light-induced cultured photoreceptor cell death. Representative fluorescence microscopy images showing nuclear staining for Hoechst 33342 and PI after 27 hours of H₂O₂ (0.3 mM) treatment (A) or 24 hours of light irradiation (2,500 lx) (C, E). (B, D, F): The number of cells exhibiting PI fluorescence was counted, and positive cells were expressed as the percentage of PI- to Hoechst 33342-positive cells. (B): ASC-CM exerted a protective effect against H₂O₂-induced cell death in a concentration-dependent manner. (D): ASC-CM, but not MA-CM, reduced visible light-induced cell death. (F): Concentrated ASC-CM reduced light-induced cell death in a concentration-dependent manner. Scale bars = 50 µm. Data are shown as mean ± SEM (n = 6). *, p < .05 versus vehicle; **, p < .01; ##, p, < .01 versus control; §, p, < .05. Abbreviations: ASC-CM, adipose-derived stem cell-conditioned medium; Con, control; H₂O₂, hydrogen peroxide; MA-CM, mature adipocyte-conditioned medium; PI, propidium iodide; Veh, vehicle.