Mononuclear cells were isolated from sow blood or colostrum, phenotyped with swine specific γδ, CD8, or CD4 monoclonal antibodies (n=16), and analyzed via flow cytometry. (A) Representative flow cytometric analysis of T lymphocyte subpopulations in sow blood (dark gray) and colostrum (black); light gray represents unstained control. (B) Percentages of T lymphocyte subsets in sow blood and colostrum, n=16. (C) Percentages of T lymphocyte subsets in blood from piglets prior to colostrum ingestion (pre-suckling) and again at 24 h (after-suckling), n=34.Variance is given by standard error. Samples with same letters are significantly different; for samples marked “a,” p=0.001, for samples marked “b” p=0.0001.