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. 2014 Jan 13;14:21. doi: 10.1186/1471-2229-14-21

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Copyright © 2014 Monteoliva et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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In vivo ¹⁴C-Pro consumption at phases I and II of HR. A: The ¹⁴C-Pro molecule used in the assay conserved all labeled carbons (*) after its conversion into ¹⁴C-P5C, and vice versa. B: Scheme of the experimental design applied to evaluate in vivo¹⁴C-Pro consumption in healthy and Pst-AvrRpm1-treated leaves (see Methods for details). C: PhosphorImager scan of a TLC plate including samples described in B. One representative plate from three independent biological experiments is shown. * indicates the origin of TLC plate. Pro, P5C and Glu positions, determined by ninhydrin staining, are indicated on the right. D: Densitometric analysis of the ¹⁴C-Pro content in the TLC plate using ImageJ software. Data are means ± SD of three independent experiments.