Table 1.
In vitro ProDH activity at different HR phases
| |
wild type |
p5cdh
|
||
|---|---|---|---|---|
| - T4C | + T4C | - T4C | +T4C | |
|
Control |
0.013 ± 0.001 |
nd |
0.012 ± 0.002 |
Nd |
|
Phase I |
0.25 ± 0.09* |
0.012 ± 0.001 |
0.190 ± 0.009* |
0.065 ± 0.005 |
|
Phase II |
0.29 ± 0.08* |
0.066 ± 0.009 |
0.240 ± 0.006* |
0.038 ± 0.009 |
| Phase III | 0.34 ± 0.05* | 0.053 ± 0.006 | nd | Nd |
ProDH activity was analyzed on protein extracts of uninoculated (control) and Pst-AvrRpm1-inoculated leaves of wild type and p5cdh plants. Infected leaves were evaluated at 6, 10 and 24 hours post-infection (phases I, II and III, respectively). Enzyme activity was expressed in nmol of Pro min-1 mg of total protein-1. Each value is mean ± SD of at least two independent experiments with 20 independent plants.
T4C: L -thiazolidine-4-carboxylic acid, inhibitor of ProDH.
*significant differences respect to uninfected condition (control) of the same genotype (p < 0.05, by t test).