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. 2014 Jan 13;14:21. doi: 10.1186/1471-2229-14-21

Table 2.

Amino acid content at different HR phases

 
 wild type
 p5cdh
  C I II III C I II III
Ser
 872 ± 129
 840 ± 160
 948 ± 98
 1387 ± 150
 895 ± 96
 1013 ± 65
 891 ± 19
 820 ± 70
Arg
 7.2 ± 0.9
 11.2 ± 0.5*
 19 ± 4*
 62 ± 7*
 7.1 ± 0.9
 9.1 ± 1.2*
 19 ± 4*
 34 ± 6*
Tyr
 10 ± 1
 21 ± 1*
 34 ± 6*
 78 ± 7*
 11 ± 1
 17 ± 1*
 39 ± 3*
 33 ± 5*
Val
 59 ± 8
 173 ± 17*
 171 ± 14*
 440 ± 76*
 59 ± 4
 102 ± 10*
 188 ± 22*
 223 ± 44*
Phe
 28 ± 5
 98 ± 6*
 108 ± 22*
 272 ± 36*
 29 ± 3
 56 ± 4*
 118 ± 12*
 127 ± 27*
His
 13 ± 2
 26 ± 5*
 37 ± 6*
 87 ± 16*
 16 ± 3
 28.7 ± 1.4*
 35 ± 5*
 38 ± 4*
Ile
 17 ± 4
 56 ± 10*
 58 ± 12*
 228 ± 45*
 20 ± 2
 40 ± 3*
 78 ± 7*
 50 ± 7*
Lys
 9 ± 2
 20 ± 4*
 23 ± 5*
 92 ± 19*
 10 ± 2
 18 ± 4*
 38 ± 5*
 #
Leu
 25 ± 3
 95 ± 8*
 115 ± 24*
 408 ± 59*
 22 ± 3
 51 ± 9*
 128 ± 9*
 169 ± 36*
Gly
 230 ± 38
 280 ± 48*
 368 ± 42*
 708 ± 31*
 284 ± 17
 259 ± 7*
 588 ± 32*
 531 ± 74*
Asn
 75 ± 16
 124 ± 5*
 145 ± 20*
 134 ± 24*
 90 ± 15
 95 ± 18
 128 ± 19
 158 ± 17*
Thr
 300 ± 43
 497 ± 35*
 449 ± 46*
 733 ± 74*
 366 ± 55
 459 ± 32*
 464 ± 60*
 #
Ala
 337 ± 46
 433 ± 87*
 391 ± 45*
 618 ± 28*
 318 ± 23
 410 ± 31*
 425 ± 15*
 299 ± 57
GABA
 19 ± 2
 30 ± 3*
 164 ± 23*
 11 ± 1*
 13 ± 3
 19 ± 4*
 291 ± 37*
 4 ± 1*
Glu
 693 ± 60
 701 ± 49
 812 ± 89
 279 ± 49*
 742 ± 83
 833 ± 77
 684 ± 51
 523 ± 43*
Asp
 285 ± 41
 228 ± 48
 300 ± 24
 62 ± 15*
 324 ± 8
 401 ± 12*
 202 ± 26*
 114 ± 12*
P5C
 1.3 ± 0.2
 1.4 ± 0.3
 1.4 ± 0.3
 1.6 ± 0.2
 1.2 ± 0.2
 1.3 ± 0.1
 1.9 ± 0.3*
 1.4 ± 0.3
Pro
 606 ± 106
 376 ± 74*
 572 ± 109
 1393 ± 169*
 995 ± 134 a
 951 ± 201
 941 ± 87
 1112 ± 22*
Gln 916 ± 100 1114 ± 239* 718 ± 31* 764 ± 92 785 ± 70 838 ± 89* 836 ± 36 437 ± 97*

C:uninfected samples (control); I, II, III: Pst-AvrRpm1-infected samples isolated at phases I, II or III of HR.

*Significant differences between infected and control samples of the same genotype (p < 0.05 by t test).

#Samples analyzed in different experiments containing similar values similar to those of wild type plants.

aSignificant differences regarding the wild type control value (p < 0.05 by t test).

Values are expressed as nmol/g fresh weight. Each value is mean ± SD of 3 independent experiments (10 leaves per plant, 6 plants per experiment).