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. Author manuscript; available in PMC: 2014 Jun 16.
Published in final edited form as: Nat Commun. 2013;4:2968. doi: 10.1038/ncomms3968

Figure 1. Dmpd is a nuclear factor that stimulates Bcd-dependent reporter activity in cells.

Figure 1

(a and b) FLAG-Dmpd interacts with HA-Bcd. Coimmunopreciptiation (co-IP) experiments were performed to detect the interaction between FLAG-Dmpd and HA-Bcd. Here, extracts from HEK293T cells expressing the indicated fusion proteins were subject to co-IP by the indicated antibodies, followed by Western blot detections by the indicated antibodies. Lanes 5 and 6 in these panels document the specificity of the antibodies used in our study.

(c) Bcd activity is stimulated by Dmpd. Reporter assays were performed in Drosophila S2 cells to evaluate the impact of Dmpd expression on CAT reporter activity. For each set of experiments, CAT activities were normalized to those obtained from cells without Dmpd expression (set as 100). Data shown are from three independent sets of experiments, with standard deviation (sd) shown for Dmpd-expressing cells.

(d-f) Nuclear localization of Dmpd. A plasmid expressing the HA-tagged Dmpd protein (HA-Dmpd) was transfected into S2 cells. Immunocytochemistry experiments were then performed to evaluate its sub-cellular localization through the use of the anti-HA antibody (e). To-pro-3 was used to counterstain the nucleus (d). Panel f is a merge of images shown in panels d and e. Scale bar is 10 μm.