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. 2004 Feb 27;32(4):1422–1429. doi: 10.1093/nar/gkh287

Figure 1.

Figure 1

Formation of a stable Ψ55S-RNA complex. (A) Nucleotide sequence of a stem–loop RNA employed in this study. The conserved nucleotides required to be substrates of Ψ55S are colored green. U, uridine; dU, 2′-deoxyuridine; 2′FU, 2′-fluorouridine; 5FU, 5-fluorouridine. (B) SDS gel analysis of reaction mixtures of Ψ55S and RNA under conditions indicated. (C) Effect of mutations of the conserved nucleotides in RNA on the formation of Ψ55S-RNA complex. The band indicated by an arrow is the Ψ55S-RNA complex.