Table 2.

Features of some probe design tools with respect to oligo length, location, specificity, accessibility, and Tm uniform.

	Probe length	Location	Specificity	Accessibility	Tm Uniform
Array Designer	User defined	User can choose 3’ UTR, 5’ UTR, or coding region	BLAST	N/A	N/A
Oligo Array 2.0	Optimal probe length selection within from a user defined range	Backward 3’ UTR end	BLAST	Mfold	Nearest neighbor model
Oligodb	User defined	From 5’ UTR to 3’ UTR direction	BLAST	Mfold	Nearest neighbor model based program: melting
OligoPicker	User defined within the range between 20 and 100	Close to 5’ UTR end	BLAST	Self-complementary likelihood	Schildkraut formula
PROBEWIZ	Optimal probe length selection within from a user defined range	N/A	BLAST	Unknown	N/A
Sarani	Optimal probe length selection within from a user defined range	N/A	BLAST	Unrevealed algorithm	Nearest neighbor model
Visual OMP	User defined or Optimal probe length selection	Let user choose visually	BLAST	Shows structure visually	N-stage model