Table 1. Run time studies for PT server generation, automated alignment and parsimony-based treeing.

No. of sequences	10	100	1000	10 000	25 743
PT server	–	5 s	22 s	3 min	7 min 30s
Add to alignment	4 s	38 s	6 min
Add to tree	1 min	9 min 15 s	2 h

Datasets varying with respect to the number of sequence entries were used for PT server generation. The most comprehensive of these datasets comprising 25 743 entries and 40 000 alignment positions was used as a template for inserting the specified numbers of sequences into the database alignment or tree. For treeing, 2141 alignment columns were included.