TABLE I.

Best-fit parameters that yield the model rates (lines) in Fig. 2.

	H4	H4-R45H
ΔG30	−40.7 pN nm (−9.9kB T)*	−42.0 pN nm (−10.2 kB T)*
ΔG35	−40.7 pN nm (−9.9 kB T)*	−42.0 pN nm (−10.2 kB T)*
ΔG40	−21.5 ± 1.3 pN nm (−5.2 ± 0.3kB T)	−5.6 ± 2.0 pN nm (−1.4 ± 0. 5kB T)
ΔG45	−34.1 ± 1.6 pN nm (−8.3 ± 0.4kB T)	−30.7 ± 1.1 pN nm (−7.5 ± 0.3kB T)
ΔG50	−37.2 ± 2.6 pN nm (−9.1 ± 0.6kB T)	−32.6 ± 2.2 pN nm (−7.9 ± 0.5kB T)
ΔG55	0.0 pN nm**	0.0 pN nm**
ΔG60	−6.7 ± 0.5 pN nm (−1.6 ± 0.1kB T)	0.0 pN nm**
ΔG65	−6.7 pN nm (−1.6 kB T)***	0.0 pN nm**
ΔG70	−6.7 pN nm (−1.6 kB T)***	0.0 pN nm**
δ	0.82 ± 0.37 bp	2.88 ± 0.33 bp
D	5500 ± 990 bp2s−1****	5550 ± 990 bp2s−1****
ΔG‡	−128 ± 25 pN nm (32.9 ± 7.3kB T)	N.A.
x‡	8.3 ± 1.7 nm	N.A.

^*Parameters constrained to ensure that the unwinding and rewinding rates are equal at F^* and equal to each other.

^**Parameters fixed at zero.

^***Fixed equal to ΔG₆₀.

^****Rates for nucleosomes containing H4 and nucleosomes containing H4-R45H were fit simultaneously using the same value of D for both data sets. The quoted errors correspond to the standard errors produced by Mathematica’s NonLinearModelFit via the ParameterErrors property.