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. Author manuscript; available in PMC: 2014 Nov 26.
Published in final edited form as: Biochemistry. 2013 Nov 14;52(47):8430–8441. doi: 10.1021/bi4010914

Figure 3.

Figure 3

2H-ESEEM spectra (open and filled circles) obtained by Fourier transformation of ESEEM data from the ternary complexes of TyrH/NO/3,5-2H- tyrosine divided by TyrH/NO/tyr. Data were collected at 9.684 GHz using the following field positions and tau values a) 190 mT, 124 ns; b) 200 mT, 116 ns; c) 225 mT, 104 ns; d) 275 mT, 84 ns; e) 300 mT, 156 ns; and f) 320 mT, 148 ns. The solid lines in each frame are best fit ESEEM simulations to the data points represented by the filled circles with error bars using the following spin Hamiltonian parameters: gn = 0.8574, T = 0.18 MHz, βhf = 26°, γhf = 0°, e2qQ/h = 0.27 MHz, βnqi = 62° and γnqi = 38°.