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. Author manuscript; available in PMC: 2014 Nov 26.
Published in final edited form as: Biochemistry. 2013 Nov 14;52(47):8430–8441. doi: 10.1021/bi4010914

Table 2.

2H – ESEEM Analysis Results

sample T
(MHz)
reff (Å) βhf(o) e2qQ/h
(MHz)
βnqi(o) |γnqi−γhf|
χν2
TyrH {2H-tyr} 0.18 ± 0.01 4.1 ± 0.1 26 ± 5° 0.27 ± 0.05 62 ± 12° 38 ± 20° 1.2
TyrH {2H-tyr, 6-MPH4} 0.12 ± 0.01 4.7 ± 0.2 94 ± 10° 0.34 ± 0.06 69 ± 10° 20 ± 80° 2.2
E332A {2H-tyr, 6-MPH4} 0.12 ± 0.01 4.7 ± 0.2 89 ± 9° 0.29 ± 0.05 44 ± 16° 14 ± 43° 1.2
TyrH {tyr, 2H-6-MPH4} 0.14 ± 0.01 4.4 ± 0.2 66 ± 5° 0.22 ± 0.06 70 ± 57° 26 ± 90° 1.2
E332A {tyr, 2H-6-MPH4} 0.14 ± 0.01 4.4 ± 0.2 65 ± 5° 0.20 ± 0.06 66 ± 60° 18 ± 90° 1.0
TyrH {2H-6-MPH4} < 0.06 >5.9