Figure 4.

Disintegration activity of the integrases. (A) Reactions were performed as described under Materials and Methods. The different INs (5 pmol) were incubated with the DNA disintegration substrate (1 pmol) for 1 h at 37°C. (B) Products were separated on denaturing 12% polyacrylamide gel and autoradiographed. S corresponds to the dumbbell substrate and P to the disintegrated product. Integrases are named by the amino acid sequence present in the V₁₅₁E₁₅₂S₁₅₃ region.