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. 2004 Mar 3;32(4):1527–1538. doi: 10.1093/nar/gkh298

Table 1. Sequence of the V151 E152S153 region of the selected integrases.

IN No. of selected clones V151E152S153 sequence Nucleotide sequence
WT IN 5 VES GTA GAA TCT
d1 3 TEQ ACT GAG CAA
d46 2 LEL CTG GAA CTG
d34 8 LVF TTG GTA TTT
d37 3 AMA GCT ATG GCG
d30 2 YVP TAC GTT CCA
r14 2 HST CAT TCT ACC
r24 1 ALT GCT TTA ACT
r1 1 KLT AAA CTA ACT
r9 3 SSP TCG AGC CCG
r17 2 HGL CAC GGC CTT
r21 1 VNR GTG AAC AGA
r22 1 ESN GAA TCA AAT
r34 6 TSM ACT AGC ATG
r36 3 TFC ACT TTT TGC

Sequence selection was carried out as described in Materials and Methods. The 43 selected mutants were distributed in the 15 families presented here. The names of the IN mutants were chosen arbitrarily: d means that they were selected in the diploid yeast strain and r in the haploid RAD52 deficient strain. The number of clones in each family and both nucleotide and amino acid sequences corresponding to the V151E152S153 region are indicated.