Table 1. Sequence of the V151 E152S153 region of the selected integrases.
IN | No. of selected clones | V151E152S153 sequence | Nucleotide sequence |
---|---|---|---|
WT IN | 5 | VES | GTA GAA TCT |
d1 | 3 | TEQ | ACT GAG CAA |
d46 | 2 | LEL | CTG GAA CTG |
d34 | 8 | LVF | TTG GTA TTT |
d37 | 3 | AMA | GCT ATG GCG |
d30 | 2 | YVP | TAC GTT CCA |
r14 | 2 | HST | CAT TCT ACC |
r24 | 1 | ALT | GCT TTA ACT |
r1 | 1 | KLT | AAA CTA ACT |
r9 | 3 | SSP | TCG AGC CCG |
r17 | 2 | HGL | CAC GGC CTT |
r21 | 1 | VNR | GTG AAC AGA |
r22 | 1 | ESN | GAA TCA AAT |
r34 | 6 | TSM | ACT AGC ATG |
r36 | 3 | TFC | ACT TTT TGC |
Sequence selection was carried out as described in Materials and Methods. The 43 selected mutants were distributed in the 15 families presented here. The names of the IN mutants were chosen arbitrarily: d means that they were selected in the diploid yeast strain and r in the haploid RAD52 deficient strain. The number of clones in each family and both nucleotide and amino acid sequences corresponding to the V151E152S153 region are indicated.