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. 2013 Sep 25;42(2):e8. doi: 10.1093/nar/gkt865

Table 2.

Sequencing metrics of SI- and LI-WGS libraries for patients 1, 2 and 3

Metric Patient 1
Patient 2
Patient 3
SI LI SI LI SI LI
Total amount of data generated (GB) 275.6 73.6 285.3 67.4 341.7 74.2
Q30 data generated (GB) 196.5 60.9 261.8 54.2 307.1 60.9

Normal Tumor Normal Tumor Normal Tumor Normal Tumor Normal Tumor Normal Tumor

Number of flowcell lanes sequenced 5 5 1 1 4 4 1 1 4 4 1 1
Read length 102 102 101 101 104 104 101 101 104 104 101 101
Average cluster density (K/mm2) 958.4 756.0 705.9 712.5 819.3 753.5
Average PF cluster density (%) 65.0 84.4 88.0 82.5 92.1 85.3
Total number of reads 1 482 053 066 1 214 032 574 330 467 210 338 562 714 1 512 625 416 1 231 472 418 289 650 634 311 034 636 1 665 626 188 1 617 770 394 402 134 806 270 012 982
Total number of mapped reads 1 387 438 027 1 110 401 730 309 910 146 318 045 021 1 426 314 916 1 158 550 623 270 915 362 289 775 606 1 574 312 575 151 7223 113 376 993 087 253 457 102
% mapped reads 93.62 91.46 93.78 93.94 94.29 94.08 93.53 93.17 94.52 93.78 93.75 93.87
Average mapped sequence coveragea 45.11 36.10 9.98 10.24 47.28 38.41 8.72 9.33 52.19 50.30 12.14 8.16
Average mapped physical coveragea 144.48 116.03 83.86 86.20 131.40 108.19 73.38 78.33 146.37 140.13 108.28 72.58

aSequence and physical coverages were calculated using all data generated. SI libraries were sequenced across five flowcell lanes, whereas LI libraries were sequenced across one flowcell lane.