Table 2.
Metric | Patient 1 |
Patient 2 |
Patient 3 |
|||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
SI | LI | SI | LI | SI | LI | |||||||
Total amount of data generated (GB) | 275.6 | 73.6 | 285.3 | 67.4 | 341.7 | 74.2 | ||||||
Q30 data generated (GB) | 196.5 | 60.9 | 261.8 | 54.2 | 307.1 | 60.9 | ||||||
Normal | Tumor | Normal | Tumor | Normal | Tumor | Normal | Tumor | Normal | Tumor | Normal | Tumor | |
Number of flowcell lanes sequenced | 5 | 5 | 1 | 1 | 4 | 4 | 1 | 1 | 4 | 4 | 1 | 1 |
Read length | 102 | 102 | 101 | 101 | 104 | 104 | 101 | 101 | 104 | 104 | 101 | 101 |
Average cluster density (K/mm2) | 958.4 | 756.0 | 705.9 | 712.5 | 819.3 | 753.5 | ||||||
Average PF cluster density (%) | 65.0 | 84.4 | 88.0 | 82.5 | 92.1 | 85.3 | ||||||
Total number of reads | 1 482 053 066 | 1 214 032 574 | 330 467 210 | 338 562 714 | 1 512 625 416 | 1 231 472 418 | 289 650 634 | 311 034 636 | 1 665 626 188 | 1 617 770 394 | 402 134 806 | 270 012 982 |
Total number of mapped reads | 1 387 438 027 | 1 110 401 730 | 309 910 146 | 318 045 021 | 1 426 314 916 | 1 158 550 623 | 270 915 362 | 289 775 606 | 1 574 312 575 | 151 7223 113 | 376 993 087 | 253 457 102 |
% mapped reads | 93.62 | 91.46 | 93.78 | 93.94 | 94.29 | 94.08 | 93.53 | 93.17 | 94.52 | 93.78 | 93.75 | 93.87 |
Average mapped sequence coveragea | 45.11 | 36.10 | 9.98 | 10.24 | 47.28 | 38.41 | 8.72 | 9.33 | 52.19 | 50.30 | 12.14 | 8.16 |
Average mapped physical coveragea | 144.48 | 116.03 | 83.86 | 86.20 | 131.40 | 108.19 | 73.38 | 78.33 | 146.37 | 140.13 | 108.28 | 72.58 |
aSequence and physical coverages were calculated using all data generated. SI libraries were sequenced across five flowcell lanes, whereas LI libraries were sequenced across one flowcell lane.