Figure 1.

TAC-induced hypertrophy is associated with the release of Ezh2 at ANP and BNP genes. (A) Pressure overload-induced cardiac hypertrophy in mice was attenuated by TSA administration. Image of Sham (control) and TAC hearts as well as reduced left arterial thrombus following TSA administration (black bar = 5 mm). (B) Hypertrophy-induced expression of miR-208b and pri-miR-208b transcripts in the LV of TAC mice was attenuated by TSA administration; *P < 0.001. (C) ChIP for Ezh2 enzyme followed by real-time qPCR for Anp and Bnp promoters shows reduced binding of Ezh2 in TAC mice; *P < 0.02. (D) ChIP for H3K27m3 followed by real-time qPCR for Anp and Bnp promoters shows reduced H3K27m3 enrichment in TAC mice. (n = 4); *P < 0.02.