Figure 7.

Schematic representation of the α/β MHC gene regulating events at the intergenic bdP mediated by ncRNA. Cardiac hypertrophy is associated with H3K27m3 of intergenic bdP by the PcG methyltransferase protein, Ezh2 and suppression of coding α-MHC and non-coding AS β-MHC gene expression. Increased expression of pri-miR-208b is associated with Ezh2 interaction at the bdP in cardiac hypertrophy. HDAC inhibition by TSA attenuates cardiac hypertrophy by restoring the expression of α-MHC and AS β-MHC genes and this is associated with hyperacetylation of the intergenic bdP as well as the release of Ezh2/pri-miR-208b complex. Restoration of AS β-MHC expression results in chromatin binding and inhibition of sense β-MHC transcription.