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. 2013 Oct 8;42(2):1311–1325. doi: 10.1093/nar/gkt899

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Analytical ultracentrifugation analysis of NP/histone complexes. (A) Sedimentation velocity analysis of eNP/H3-H4 complexes at 1/1 and 1/2 molar ratios, and (B) of the eNP/octamer (1/0.5 molar ratio) complex. (C) Sedimentation equilibrium analysis of the eNP/octamer (1/0.5 molar ratio) complex. The top panel shows the absorbance at 230 nm as a function of the square of the radial distance of the sample at any position within the cell (r) minus the square of the radial position at a reference point (r₀) (r²-r₀²). The continuous lines in these plots were obtained by fitting the experimental data to a single ideal species with Mr of ∼307 kDa. The bottom plot shows X² residuals as a function of r-r₀² for the best fit (solid lines). The run was performed at 4°C and 8000 (circles) and 12 000 (diamonds) rpm.